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bapta am  (Selleck Chemicals)


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    Selleck Chemicals bapta am
    Bapta Am, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bapta+am/BAPTA-AM/pm42521040-210-15-16
    Average 94 stars, based on 112 article reviews
    bapta am - by Bioz Stars, 2026-10
    94/100 stars

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    other:

    Article Title: BH4 Oxidation-Derived H 2 O 2 Activates ERK1/2 Signaling via B-Raf in Rat Dorsal Root Ganglion Neurons.
    Article Snippet: For stimulations, BH4 (Sigma, cat. no. T4425), NGF (Alomone labs, cat. no. N- 240), OSM (Peprotech, cat. no. 400- 36), ascorbic acid (Sigma, cat. no. A4403), phenyl- alpha- tert- butyl nitrone (PBN) (Enzo, cat. no. ALX- 430- 082- G001), BH2 (MCE MedChemExpress, cat. no. HY- W008646), H2O2 (Applichem, cat. no. A2726,1000), catalase (Sigma, cat. no. C30- 100MG), superoxide dismutase (Sigma, cat. no. S9697- 15KU), 4- hydrox y- 2,2,6,6- tetramethylpiperidin- 1- oxyl (Tempol) (Selleckchem, cat. no. S2910), ML171 (also 2- APT) (Tocris, cat. no. 4653), AITC (Sigma, cat. no. 377430), capsaicin (Sigma, cat. no. M2028), L- NAME (Cayman, 51298- 62- 5, cat. no. 80210), LNNA (Tocris, cat. no. 0664), L- NMMA (Tocris, cat. no. 0771), ruthenium red (Abcam, cat. no. ab120264), 2- Aminoethyl diphenylborinate (2- APB) (Calbiochem, cat. no. 100065), 14714159, 2025, 11, at Prov Indonesia, on [07/11/2025]

    Recombinant:

    Article Title: PIEZO1-mediated endometrial decidualization is disrupted in miscarriage through BECN1-dependent autophagy
    Article Snippet: Stromal cells were treated with Yoda1 (800nM, HY-18723, MedChemExpress) for 48-hour during decidualization period. .. Inhibitors and recombinant proteins, including BAPTA-AM (10 μM, S7534, Selleck, Shanghai, China), KN93 (5 μM, S6787, Selleck, Shanghai, China), U0126 (25 μM, S1102, Selleck, Shanghai, China), and Tat-BECLIN1 (2 μM, S8595, Selleck, Shanghai, China), were applied 1 hour prior to Yoda1, respectively. .. Human endometrial stromal cells (ATCC, CRL-4003TM) were seeded in 12-well plates and cultured in AR TI CL E IN P RE SS DMEM/F12 supplemented with 10% charcoal-treated fetal bovine serum (cFBS, Biological Industries) until approximately 80% confluence at 37 °C and 5% CO2.

    Article Title: Wnt/β-catenin signaling restrains developmental beige adipocyte thermogenesis and its inhibition imprints long-term energy expenditure
    Article Snippet: .. For inhibition and activation experiments, once full adipogenic differentiation was confirmed, cells were treated with each of the following molecules for 4 days: MSAB (S6901,Selleck, 10 uM), BAPTA-AM (S7534, Selleck, 5 uM), Box5 (P1216, Selleck, 100 uM), Wnt5a recombinant protein (HY-P704313, MCE, 100 ng/mL) with the exception of Rosiglitazone (S2556, Selleck, 2 uM), which was added on the first day of adipogenic induction in SVFs. ..

    Translocation Assay:

    Article Title: NFATc1 Mediates LIPUS-Induced Osteoblast Differentiation through P2X7 in MC3T3-E1 Cells
    Article Snippet: .. To investigate the role of intracellular Ca2+ in the nuclear translocation of NFATc1 following LIPUS stimulation, we measured the nuclear translocation of NFATc1 in MC3T3-E1 cells after LIPUS stimulation and 5μM BAPTA-AM (Selleck Chemicals) treatment12). ..

    Inhibition:

    Article Title: Wnt/β-catenin signaling restrains developmental beige adipocyte thermogenesis and its inhibition imprints long-term energy expenditure
    Article Snippet: .. For inhibition and activation experiments, once full adipogenic differentiation was confirmed, cells were treated with each of the following molecules for 4 days: MSAB (S6901,Selleck, 10 uM), BAPTA-AM (S7534, Selleck, 5 uM), Box5 (P1216, Selleck, 100 uM), Wnt5a recombinant protein (HY-P704313, MCE, 100 ng/mL) with the exception of Rosiglitazone (S2556, Selleck, 2 uM), which was added on the first day of adipogenic induction in SVFs. ..

    Activation Assay:

    Article Title: Wnt/β-catenin signaling restrains developmental beige adipocyte thermogenesis and its inhibition imprints long-term energy expenditure
    Article Snippet: .. For inhibition and activation experiments, once full adipogenic differentiation was confirmed, cells were treated with each of the following molecules for 4 days: MSAB (S6901,Selleck, 10 uM), BAPTA-AM (S7534, Selleck, 5 uM), Box5 (P1216, Selleck, 100 uM), Wnt5a recombinant protein (HY-P704313, MCE, 100 ng/mL) with the exception of Rosiglitazone (S2556, Selleck, 2 uM), which was added on the first day of adipogenic induction in SVFs. ..



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    NETs disrupt calcium-NFAT signaling to suppress activation in CD4 + T cells. A PCA of transcriptomes from untreated CD4 + T cells and NETs-treated CD4 + T cells at 3 and 7 days. CON = Control (untreated CD4 + T cells). The RNA-seq culture condition was anti-CD3/CD28-stimulated. B Heatmap of differentially expressed genes (DEGs) among the three groups. Transcriptional changes in (A) and (B) correspond to CD4. + T cells stimulated with SS-NETs (isolated from sepsis patient serum-induced neutrophils) vs. untreated control cells. C IL-2 concentrations in cell culture supernatants from anti-CD3/CD28 (5 μg/mL)-activated Jurkat cells after 3 days of treatment with varying NETs, measured by enzyme-linked immunosorbent assay (ELISA). D Expression level of NFATc1, ZAP70, p-ZAP70, P65, p-P65, ERK, p-ERK in Jurkat cells following NETs treatment, detected by Western blot. Cells were stimulated with anti-CD3/CD28 antibodies and exposed to SS-NETs, LPS-NETs, PMA-NETs, or vehicle control for 72 h. E Nuclear translocation of NFATc1 protein, assessed by immunofluorescence. co-stained for DNA (blue) and NFATc1 (green). NC: negative control; SS: septic serum. Scale bar: 50 μm. 40 × magnification observation. F Calcineurin activity in anti-CD3/CD28 (5 μg/mL)-activated Jurkat cells after 3 days of treatment with varying NETs, measured by ELISA. G Basal intracellular calcium levels in Jurkat cells treated with varying NETs, determined by ratiometric analysis, NC: negative control. H The level and peak of calcium ion influx after BAPTA-AM chelates intracellular calcium detected by flow cytometry. NC: negative control; SS: septic serum. *, P < 0.05; ***, P < 0.001

    Journal: Molecular Medicine

    Article Title: NETs drive CD4⁺ T cell dysfunction in sepsis via mitochondrial ROS and impaired calcium signalling

    doi: 10.1186/s10020-026-01607-0

    Figure Lengend Snippet: NETs disrupt calcium-NFAT signaling to suppress activation in CD4 + T cells. A PCA of transcriptomes from untreated CD4 + T cells and NETs-treated CD4 + T cells at 3 and 7 days. CON = Control (untreated CD4 + T cells). The RNA-seq culture condition was anti-CD3/CD28-stimulated. B Heatmap of differentially expressed genes (DEGs) among the three groups. Transcriptional changes in (A) and (B) correspond to CD4. + T cells stimulated with SS-NETs (isolated from sepsis patient serum-induced neutrophils) vs. untreated control cells. C IL-2 concentrations in cell culture supernatants from anti-CD3/CD28 (5 μg/mL)-activated Jurkat cells after 3 days of treatment with varying NETs, measured by enzyme-linked immunosorbent assay (ELISA). D Expression level of NFATc1, ZAP70, p-ZAP70, P65, p-P65, ERK, p-ERK in Jurkat cells following NETs treatment, detected by Western blot. Cells were stimulated with anti-CD3/CD28 antibodies and exposed to SS-NETs, LPS-NETs, PMA-NETs, or vehicle control for 72 h. E Nuclear translocation of NFATc1 protein, assessed by immunofluorescence. co-stained for DNA (blue) and NFATc1 (green). NC: negative control; SS: septic serum. Scale bar: 50 μm. 40 × magnification observation. F Calcineurin activity in anti-CD3/CD28 (5 μg/mL)-activated Jurkat cells after 3 days of treatment with varying NETs, measured by ELISA. G Basal intracellular calcium levels in Jurkat cells treated with varying NETs, determined by ratiometric analysis, NC: negative control. H The level and peak of calcium ion influx after BAPTA-AM chelates intracellular calcium detected by flow cytometry. NC: negative control; SS: septic serum. *, P < 0.05; ***, P < 0.001

    Article Snippet: Calcium influx was evaluated by flow cytometry, intracellular calcium was chelated with BAPTA-AM (HY-100545, MCE) and then the above experimental steps were followed.

    Techniques: Activation Assay, Control, RNA Sequencing, Isolation, Cell Culture, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Translocation Assay, Immunofluorescence, Staining, Negative Control, Activity Assay, Analysis, Flow Cytometry